Transfer a quantity of powdered Tablets, equivalent to about 50 mg of nadolol, to a conical flask. Add 10 mL of 0.1 N hydrochloric acid, stir for 30 minutes, using a magnetic stirrer, and place in an ultrasonic bath for an additional 30 minutes. Centrifuge, and use the supernatant for the test solution. Apply, as streaks, 100 µL of the test solution and 100 µL of a Standard solution of USP Nadolol RS
in 0.1 N hydrochloric acid having a concentration of about 5 mg per mL to a thin-layer chromatographic plate (see Chromatography 621
) coated with a 0.25-mm layer of chromatographic silica gel mixture. Develop the chromatogram in a solvent system consisting of acetone, chloroform, and 2 N ammonium hydroxide (8:1:1) until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the developing chamber, allow the solvent to evaporate, and examine the chromatogram under short-wavelength UV light: the RF
value of the principal spot obtained from the test solution corresponds to that obtained from the Standard solution.
0.01 N hydrochloric acid; 900 mL.
Determine the amount of C17
dissolved, employing the procedure set forth in the Assay
, except to prepare the Mobile phase
using 560 mL of methanol and 1440 mL of water instead of 700 mL and 1300 mL, respectively, and adjusting with 0.1 N hydrochloric acid to a pH of 2.5. Use filtered portions of the solution under test, suitably diluted with Medium
, if necessary, in comparison with a Standard solution having a known concentration of USP Nadolol RS
in the same Medium
Not less than 80% (Q) of the labeled amount of C17H27NO4 is dissolved in 50 minutes.
Prepare a filtered and degassed mixture of 700 mL of methanol and 1300 mL of water containing 5.84 g of sodium chloride and 1.0 mL of 0.1 N hydrochloric acid.
Dissolve an accurately weighed quantity of USP Nadolol RS
in Mobile phase
to obtain a solution having a known concentration of about 0.2 mg per mL.
Weigh and finely powder not less than 20 Tablets. Weigh accurately a portion of the powder, equivalent to about 20 mg of nadolol, and transfer to a 100-mL volumetric flask. Add about 75 mL of Mobile phase, place in an ultrasonic bath for 15 minutes, shaking intermittently, add Mobile phase to volume, and mix. Clarify the solution by filtration or centrifugation.
(see Chromatography 621
)The liquid chromatograph is equipped with a 220-nm detector and a 4.6-mm × 25-cm column that contains packing L16. The flow rate is about 1 mL per minute. Chromatograph replicate injections of the Standard preparation
, and record the peak responses as directed for Procedure:
the relative standard deviation is not more than 2.0%; and the tailing factor for the nadolol peak is not more than 3.
Separately inject equal volumes (about 20 µL) of the Standard preparation
and the Assay preparation
into the chromatograph, record the chromatograms, and measure the responses for the major peaks. Calculate the quantity, in mg, of nadolol (C17
) in the portion of Tablets taken by the formula:
100C(rU / rS),
in which C
is the concentration, in mg per mL, of USP Nadolol RS
in the Standard preparation;
are the peak responses obtained from the Assay preparation
and the Standard preparation